Dendritic cells (DCs) that control immune responses were recently found to capture and transport HIV from the mucosal area to remote lymph nodes (1), where DCs hand over HIV to CD4+ T lymphocytes.
DCs also amplify the amount of virus and extend the duration of viral infectivity.
Multiple strains of HIV-1, HIV-2 and SIV bind to DCs via DC-SIGN (2).
ICAM-3 is the natural ligand for DC-SIGN (3).
A DC-SIGN homologue (termed DC-SIGNR, L-SIGN, and DC-SIGN2) was identified recently (4–8).
DC-SIGN forms a novel gene family with DC-SIGNR and many alternatively spliced isoforms of DC-SIGN and DC-SIGNR (8).
The expression of DC-SIGN was found in mucosal tissues including placenta, small intestine, and rectum.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:Western blot: 1-2ug/ml detects GST-DC-SIGN fusion protein expressed in E.
coli in the absence and presence of inducing reagent IPTG with anti-DC-SIGN at 0.5ug/ml
Immunohistochemistry: 10ug/mlOptimal dilutions to be determined by the researcher.
Positive control: Human placenta lysate
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 0.02% sodium azide.
Specificity：Recognizes human DC-SIGN at ~ 44kD.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide (CSRDEEQFLSPAPATPN PPPA) corresponding to aa384 to 404 of human DC-SIGN, C-terminal (1).
