Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands.
Cell death signals are transduced by death domain (DD) death effector domain (DED), and caspase recruitment domain (CARD) containing molecules.
Several molecules including caspases and adaptor FADD contain DEDs.
A novel protein that interacts with DED of caspase-8 and –10, and FADD was identified recently and designated DEDAF for DED associated factor (1).
DEDAF is identical to the transcriptional repressor RYBP (2).
DEDAF/RYBP is expressed in multiple tissues and cell lines.
DEDAF interacts with FADD and augments the formation of CD95/FADD/capase-8 complexes at the cell membrane, and interacts with DED-containing DNA biding protein (DEDD) in the nucleus indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis.
Applications: Suitable for use in Western Blotting.
Other applications not tested.
Recommended Dilution: 0.5-1ug/ml
Optimal dilutions to be determined by the researcher.
Human A549 or HepG2 cell lysate can be used as a positive control.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Immunoaffinity purified IgG
Form：100ug in 200ul of PBS, 0.02% sodium azide.
Specificity：A band at approximately 32kD can be detected. It is human, mouse and rat reactive.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide (TPKGDMSAVNDESF) corresponding to amino acids 215 to 228 of human DEDAF (1,2). The sequence is identical to that of mouse origin (2).
