Applications:Suitable for use in Western Blot, ELISA and Immunohistochemistry.
Other applications have been tested.
Recommended Dilutions:Immunohistochemistry: 50ug/ml stains PLP fixed cultured cells known to express the Muscarinic Receptor M4
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Purified
Purity:Purified by ammonium sulfate precipitation, dialysis and chromatography.
Form:Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 40% glycerol.
Specificity:Shown to be specific for the COOH terminal of the M4 receptor. Crossreactivity: M4 Muscarinic Receptor (aa469-478): 100%, M4 Muscarinic Receptor: ~70%. No reacivity is observed with M1-M3, M5 (full receptors), M1 (aa451-460), M2 (aa457-466), M3 Muscarinic Receptor (aa580-589), M5 (aa519-531).
Isotype:IgG
Calc Applications Abbrev:E IHC
Calc Crossreactivity:Hu
Immunogen:Peptide analogue of the carboxyl terminal of the M4 receptor covalently attached onto a carrier protein.