The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors.
The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration and potassium channel mediation.
Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system.
The muscarinic cholinergic receptor 1 is involved in mediation of vagally-induced bronchoconstriction and in the acid secretion of the gastrointestinal tract.
The gene encoding this receptor is localized to 11q13.
Applications:Suitable for use in Western Blot, ELISA and Immunohistochemistry.
Other applications have been tested.
Recommended Dilution:Western Blot: 20ug/ml using whole rat brain homogenateImmunohistochemistry: 5ug/ml using ABC techniques has been found to stain PLP fixed rat brain sections known to express the muscarinic M1 receptor
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified by precipitation, dialysis and chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA.
Specificity：Recognizes human and rat Muscarinic Receptor M1. Crossreactivity: M1 (aa451-460): 100%, M1 (full receptor): 60%. No reacivity is observed with M2-M5 (full receptors), M2 (aa457-466), M3 (aa580-589), M4 (aa469-478) or M5 (aa519-531).
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：Peptide analogue of the carboxyl terminal of the M1 receptor covalently attached onto a carrier protein.
