Dopamine is an endogenous catecholamine that influences many cellular activities, including behavior, hormone synthesis and release, blood pressure and intracellular ion transport.
A family of at least 5 Dopamine Receptors (DR) genes, D1-D5, have been identified based upon the amino acid identity, pharmacological specificity and effector responses.
DR have been classified into either the D1-like (D1, D1B and D5) or D2-like (D2, D3 and D4).
The two isoforms of D2R, D2 long (D2L) and short (D2S), are encoded by splice variants of a single gene and differ only by the presence of an additional 29aa in the intracellular domain 3 of the D2 long form.
It may play a role in the coupling of the receptor to G-proteins.
All members of this family have similar structure and contain 7 putative transmembrane domains.
A given cell or tissue may express more than one DR.
Specific radioligands do not exist that can differentiate between these DR.
Therefore, specific antibodies are needed to distinguish, localize, and document changes in DR levels in cells and tissues under various normal and pathological conditions.
Dopamine D4 Receptor is a 467aa, G-protein coupled and transmembrane receptor protein.
Applications:Suitable for use in Western Blot, ELISA and Immunocytochemistry.
Other applications not tested
Recommended Dilutions:Immunocytochemistry: 1:10,000 stains specific cells in various regions of PLP fixed rat brain sections This includes the glomeruli of the olfactory bulb, staining of cortex layer 4 and dendritic staining in cortex layers 2 and 3.
In layer 5 of the cortex, apical dendrites of many neurons were stained.
In the hippocampus, diffuse immunoreactivity was observed in the molecular layer of the dentate gyrus, with fine dots associated with many neurons in the granule cell layer.
Western Blot: 1:500 using whole rat brain homogenate produced a band at ~48-53kD
Optimal dilutions to be determined by the researcher
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile PBS, 10mg/ml BSA.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitute with 100ul sterile PBS, 10mg/ml BSA.
