Dopamine is an endogenous catecholamine that influences many cellular activities, including behavior, hormone synthesis and release, blood pressure and intracellular ion transport.
A family of at least 5 Dopamine Receptors (DR) genes, D1-D5, have been identified based upon the amino acid identity, pharmacological specificity and effector responses.
All members of this family have similar structure and contain 7 putative transmembrane domains.
A given cell or tissue may express more than one DR.
Specific radioligands do not exist that can differentiate between these DR.
Therefore, specific antibodies are needed to distinguish, localize and document changes in DR levels in cells and tissues under various normal and pathological conditions.
Applications:Suitable for use in Immunohistochemistry, Western Blot, and ELISA.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1:1000 (whole rat brain homogenate resulted in a single band being detected at 50kD)ELISA: 1:5000.
Immunohistochemistry: 1:5000.
(staining cells in various regions of PLP fixed rat brain sections, inlcuding Medial septum, Nucleus accumbens, Dentate gyrus, Globus pallidus, Medial forebrain bundle, Cortex regions 1-3, Substantia Nigra reticulata, and the Ventral tegmental area.
)Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile PBS, 10mg/ml BSA.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ul
Host：rabbit
Grade：Serum
Purity：Serum
Form：Supplied as a lyophilized powder. Reconstitute with 100ul sterile PBS, 10mg/ml BSA or with additional buffer for more dilute antisera.
