Hepatitis B is an infection of the liver caused by the hepatitis B virus.
Approximately 5-10% of adults and 90% of babies who are infected with HBV will go on to carry the virus for the rest of their lives.
These people will pass the virus onto others.
HBV is excreted in body fluids such as semen, saliva, blood and urine in persons with acute or chronic infection.
The route of transmission can include homosexual or heterosexual activity, blood-borne exposure (needles, transfusion), mother-infant, close personal contact and even by consuming contaminated food or water.
Thus, Hepatitis B has become a major public health concern.
When HBV invades the body it causes liver damage through induction of auto-immunity.
Liver cell injury results from cytotoxic T cell activity rather than viral cytotoxic activity.
The principal screening test for detecting current (acute or chronic) HBV infection is the identification of HBsAg, an envelope lipoprotein.
This is the first immunological marker to appear in a patient’s serum and exist in high quantities in the blood.
Patients who are HBsAg positive develop chronic persistent hepatitis (CPH) and chronic active hepatitis (CAH).
Patients with CPH usually remain in good health.
Those with CAH have progressive liver damage with the outcome being portal fibrosis, cirrhosis, or hepatocellular carcinoma.
Screening for HBsAg is recommended for all donors, pregnant women and those individuals who at high risk.
Presence of this marker is conclusive proof of HBV infection.
This immunoassay measures HBsAg through antigen-antibody interactions.
Applications:Suitable for use in Immunofluorescence.
Other applications have not been tested.
Recommended Dilutions:Direct IFA: Acetone fixation of the antigen source is recommended prior to staining
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1ml
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes Hepatitis B Surface Antigen (ad/ay). Reacts only with HBsAg including the pre-S1 epitope. Non-reactive with normal human serum.
Isotype：IgG
Calc Applications Abbrev：IF
Immunogen：Hepatitis B surface antigen purified from human serum. Mixture of subtypes ad & ay.
