Applications:Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunomicroscopy.
Other applications have not been tested.
Recommended Dilution:ELISA 1:10,000-1:50,000Western Blot: 1:1,000-1:5,000Immunohistochemistry: 1:1,000-1:2,000Optimal dilutions to be determined by the researcher.
Label: Biotin
Biotin/Protein Ratio:10-20 BAC molecules per sheelectrophoresis IgG molecule.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:2mg
Host:sheep
Source Antibody:mouse
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography, followed by solid phase adsorption to remove any unwanted reactivities.
Form:Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease Free), 0.09% sodium azide, 40% glycerol.
Specificity:Recognizes Mouse IgG heavy and light chains. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-sheep serum, mouse IgG and mouse serum.