Applications:Suitable for use in FLISA, Western Blot and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Label: Fluorescein isothiocyanate (FITC)Molecular Weight: 390DAbsorption Wavelength: 495nmEmission Wavelength: 528nmFluorochrome/Protein Ratio: 2.2 moles FITC per mole of Sheep IgG
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%) and aliquot.
Store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：2mg
Host：sheep
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 10mg/ml BSA (IgG and Protease free), 40% glycerol. Labeled with fluorescein isothiocyanate (FITC). Absorption Wavelength: 495nm.Emission Wavelength: 528nm. Fluorochrome/Protein Ratio: 2.2 moles FITC per mole of Sheep IgG.
Specificity：Recognizes Mouse IgG. Assay by IEP resulted in a single precipitin arc against anti-fluorescein, anti-sheep serum, mouse IgG and mouse serum.
