Applications:Suitable for use in Immunoblot, ELISA, Immunohistochemistry and Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Other applications not tested.
Recommended Dilution:ELISA: 1:30,000-1:150,000; I1904-83N was assayed against 1ug of mouse IgG2a in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiozoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
Optimal dilutions to be determined by the researcher.
Reconstitution:Reconstitute with 1ml sterile ddH2O or sterile 40-50% glycerol.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule.
Specificity：Recognizes mouse IgG2a. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum, mouse IgG and mouse serum. Specificty was confirmed by ELISA. Typically less than 1% crossreactivity was observed against other mouse heavy chain isotypes.
