Applications:Suitable for use in FLISA (Fluorescent) (Fluorescent), Western Blot and Immunofluorescence.
Other applications not tested.
Recommended Dilutions:Immunofluorescence: 1:1000 -1:5000FLISA (Fluorescent): 1:10,000- 1:50,000Optimal dilutions to be determined by researcher.
Label: Fluorescein isothiocyanate (FITC)Molecular Weight: 390DAbsorption Wavelength: 495nmEmission Wavelength: 528nmFluorochrome/Protein Ratio: 3.6 moles FITC per mole of rabbit IgG
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
FITC conjugates are sensitive to light.
仕様
Size：1mg
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Prepared by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC). Reconstitute with 1ml sterile ddH2O.
Specificity：Recognizes mouse IgG2a heavy chain. Crossreactivity against other mouse or human heavy or light chain isotypes is <1%.
