Applications:Suitable for use in ELISA, Immunohistochemistry, Western Blot and Immunomicroscopy.
Also suitable for other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Other applications not tested.
Recommended Dilutions:ELISA: 1:250,000Immunohistochemistry: 1:500-1:3000Western Blot: 1:1000-1-5000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA(IgG and Protease free), 0.01% sodium azide. Labeled with BiotinBiotin/Protein Ratio: 10-20 BAC molecules per rabbit IgG molecule Reconstitute with 1ml sterile ddH2O.
Specificity：Recognizes mouse IgG1. Assay by IEP resulted in a single precipitin arc against anti-biotin, anti-rabbit serum, mouse IgG and mouse serum. Specificty was confirmed by ELISA. Typically less than 1% crossreactivity was observed against other mouse heavy chain isotypes.
