Mouse IgG1 secondary antibody conjugated to FITC is available in a variety of formats.
Anti-mouse IgG1 secondary antibody conjugate is suitable for immunofluorescence microscopy, fluorescence based plate assays (FLISA (Fluorescent)) and fluorescent western blotting.
This IgG1 antibody is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms
Applications:Suitable for use in FLISA (Fluorescent) (Fluorescent), Immunofluorescence and Western Blot.
Also suitable for multiplex analysis.
Other applications not tested.
Recommended Dilutions:Immunofluorescence: 1:500-1:2500Flow Cytometry: 1:2000-1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months after receipt at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
仕様
Size：1mg
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with fluorescein isothiocyanate (FITC). Reconstitute in 1ml sterile ddH2O.
Specificity：Recognizes mouse IgG1 heavy chain. Crossreactivity against other mouse or human heavy or light chain isotypes is < 1%. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-rabbit serum, mouse IgG and mouse serum.
