Applications:Suitable for use in FLISA, Immunofluorescence and Fluorescent Western Blot..
Other applications have not been tested.
Recommended Dilutions:FLISA: 1:10,000-1:50,000Immunofluorescence: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Absorption Wavelength:495nm
Emission Wavelength:528nm
Fluorochrome/Protein Ratio:3.6 moles FITC per mole of Rabbit IgG
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in 0.125M sodium borate, 0.075M sodium chloride, 0.005M EDTA, pH 8.0, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC)
Specificity：Recognizes mouse IgM. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-rabbit serum, mouse IgM and mouse serum. No reaction was observed against mouse IgG or mouse IgA. Specificity was confirmed by ELISA at <1% crossreactivity against other mouse heavy or light chain isotypes.
