Applications:Suitable for use in FLISA, Western Blot and Immunofluorescence.
Other applications not tested.
Recommended Dilutions:Optimal dilution determined by the researcher.
Absorption Wavelength: 495nm
Emission Wavelength: 528nm
Fluorochrome/Protein Ratio: 3.7 moles FITC per mole of Rabbit IgG
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, pH 7.2, 10 mg/ml BSA (IgG and protease free), 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC). Reconstitute with sterile dH2O or 40-50% glycerol for long term storage.
Specificity：Recognzies mouse IgG (Fab)’2. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-rabbit serum, mouse IgG, mouse IgG F(ab’)2 and mouse serum. No reaction was observed against mouse IgG F(c) or human serum proteins.
