Applications:Suitable for use in Western Blot, Dot Blot, ELISA and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:ELISA: 1:500-1:3500Optimal dilutions to be determined by the researcher.
Label:Alkaline Phosphatase from Calf Intestine (MW 140kD)
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:1mg
Host:rabbit
Source Antibody:mouse
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography followed by solid phase adsorption to remove unwanted reactivities.
Form:Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (AP).
Specificity:Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Mouse IgG and Mouse Serum. No reactivity was observed against Human serum proteins.