Applications:Suitable for use in Western Blot, Dot Blot, ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1:1000-1:5000ELISA: 1:10,000-1:50,000Immunohistochemistry: 1:500-1:2500Optimal dilutions to be determined by the researcher.
Label:Horseradish Peroxidase (HRP)
Storage and Stability:Lyophilized powder is stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O prior to use.
To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ug
Host：rabbit
Source Antibody：sheep
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% Gentamicin. Labeled with horseradish peroxidase (HRP). Reconstitute with 500ul sterile ddH2O.
Specificity：Recognizes sheep IgG F(ab’)2. Assay by IEP resulted in a single precipitin arc against anti-peroxidase, anti-rabbit serum, sheep IgG, sheep IgG F(ab’)2 and sheep serum. No reaction was observed against sheep IgG F(c) or human serum proteins.
