Applications:Suitable for Immunoblot (Western or Dot Blot), ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:500 to 1:2,000; using p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：rabbit
Source Antibody：sheep
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Form：Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol.
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-rabbit serum, sheep IgG, sheep IgG F(ab’)2 and sheep serum. No reaction was observed against sheep IgG F(c).
