Application(s): Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry.
Recommended Dilution(s):Immunohistochemistry: 1:500 to 1:5000 on tissue sectionsELISA: 1:5000-1:50,000Western Blot: 1:5000-1:50,000Optimal titers for other applications should be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Freeze at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：500ug
Host：rabbit
Source Antibody：sheep
Grade：Affinity Purified
Purity：Prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption.
Form：Lyophilized in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% Thimerosal (addition of sodium azide not recommended). Labeled with horseradish peroxidase (HRP).
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Sheep IgG, Sheep IgG F(c) and Sheep Serum. No reaction was observed against Sheep IgG F(ab’)2 or Human Serum Proteins.
