Applications:Suitable for use in Western Blot, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates.
Other applications not tested.
Recommended Dilutions:ELISA: 1:15,000-1:60,000 using 1ug sheep IgG in a sandwich ELISA
Biotin/Protein Ratio:10-20 BAC molecules per Rabbit IgG molecule
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ug
Host：rabbit
Source Antibody：sheep
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography followed by solid phase adsorptions to remove any unwanted reactivities.
Form：Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin.
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum, sheep IgG, sheep IgG F(c) and sheep serum. No reaction was observed against sheep IgG F(ab’)2.
