Applications:Suitable for use in Immunoblot, ELISA, Immunohistochemistry and Immunomicroscopy Other applications not tested.
Recommended Dilution:ELISA: 1:12,000-1:60,000 using 1ug of Sheep IgG in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline- 6-sulfonic acid]) as a substrate for 30 minutes at RT
Optimal dilutions to be determined by the researcher.
Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：rabbit
Source Antibody：sheep
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin. Reconstitute with 1ml sterile, ddH2O or sterile 40-50% glycerol. Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
Specificity：Recognizes sheep IgG, H&L. Assay by IEP resulted in a single precipitin arc against anti-biotin, anti-rabbit serum, sheep IgG and sheep serum. Species Crossreactivity: Does not crossreact with human serum proteins.
