Applications:Suitable foruse in Western Blot, ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilution:ELISA: 1:1,500 to 1:6,000Western Blot: 1:500-1:2000Immunohistochemistry: 1:200-1:1000Optimal dilution determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：rabbit
Source Antibody：porcine
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (AP).
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-rabbit serum, porcine IgG, porcine IgG F(ab’) 2 and porcine serum. No reaction was observed against porcine IgG F(c).
