Applications:Suitable for use in FLISA, Western Blot and Immunofluorescence.
Other applications have not been tested.
Recommended Dilutions:FLISA: 1:10,000-1:50,000Immunofluorescence Microscopy: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Absorption Wavelength:495nm
Emission Wavelength:528nm
Fluorochrome/Protein Ratio:3.5moles FITC per mole of Rabbit IgG
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
仕様
Size：500ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease Free), 0.01% (w/v) sodium azide. Labeled with fluorescein isothiocyanate (FITC). (MW: 390 daltons)
Specificity：Recgonizes rat IgM. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-rabbit serum, rat IgM and rat serum. No reaction was observed against rat IgG or IgA. Specificity was confirmed by ELISA at <1% crossreactivity against other rat heavy or light chain isotypes.
