Applications:Suitable for use in Western Blot, Dot Blot, ELISA and Immunohistochemistry.
Other applications have not been tested.
Recommended DilutionELISA: 1:500-1:3,000.
1ug of rat IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) as a substrate for 30 min at RT.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store at 4°C before opening.
DO NOT FREEZE.
This product is stable at 4°C as an undiluted liquid.
Dilute only prior to immediate use.
Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
仕様
Size：1mg
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Form：Supplied as a liquid in 0.05M Tris chloride, 0.15M sodium chloride, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol.
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Rat IgG, Rat IgG F(c) and Rat Serum. No reaction was observed against Rat IgG F(ab’)2.
