Applications:Suitable for Western or Dot Blot, ELISA, Immunoperoxidase, Electron Microscopy and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:1000-1:50,000.
Assayed against 1ug of Rat IgG in a standard capture ELISA
Immunohistochemistry:1:500-1:2500Western Blot: 1:1000-1:10,000Optimal dilution determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Prepared by immunoaffinity chromatography followed by solid phase adsorption.
Form：Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate, 40% glycerol. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes rat IgG F(c) fragment. Does not crossreact with rat IgG F(ab’)2. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-rabbit serum, rat IgG, rat IgG F(c) and rat serum.
