Applications:Suitable for Immunoblot, ELISA, Immunohistochemistry and Immunomicroscopy.
Other applications have not been tested.
Recommended Dilutions:ELISA: 1:20,000-1:60,000 using peroxidase conjugted streptavidin and ABTS as a substrate for 30 min at RT
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin. Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule. Reconstitute with 1ml sterile, distilled, deionized water or sterile 40-50% glycerol.
Specificity：Assay by IEP resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Rat IgG and Rat Serum. No reaction was observed against Human Serum Proteins.
