Applications:Suitable for immunoblot, ELISA, immunohistochemistry and other phosphatase-antibody based enzymatic assays Other applications not tested.
Recommended Dilutions:ELISA: 1:500-1:2000 (Coat with1ug of Rat IgM)
Optimal dilutions should be determined by the researcher.
Label:Alkaline Phosphatase (Calf Intestine)
Molecular Weight: 140kD
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：goat
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (AP).
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rat IgM and Rat Serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other Rat or human heavy or light chain isotypes.
