Applications:Suitable for FLISA, Immunomicroscopy, Flow Cytometry, FACS Analysis as well as other antibody based fluorescent assays.
Other applications not tested.
Recommended Dilution:FLISA: 1:10,000-1:50,000IF Microscopy: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Absorption Wavelength:495nm
Emission Wavelength:528nm
Fluorochrome/Protein Ratio:4.8 moles FITC per mole of Goat IgG
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Host：goat
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography followed by solid phase adsorptions to remove any unwanted reactivities.
Form：Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Specificity：Recognizes rat IgM. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-goat serum, rat IgM and rat serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other rat heavy or light chain isotypes.
