Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:130,000Western Blot: 1:2000-1:10,000Immunohistochemistry: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Labeled with horseradish peroxidase (HRP).
Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
HRP conjugates are sensitive to light.
仕様
Size：1mg
Host：goat
Source Antibody：rat
Grade：Affinity Purified
Purity：Prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose.
Form：Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Labeled with Horseradish Peroxidase (HRP).
Specificity：Recognizes rat IgG, Fc. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, rat IgG, rat IgG F(c) and rat serum. No reaction was observed against rat IgG F(ab’)2.
