Applications:Suitable for Immunoblot (Western or Dot Blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:5,000-1:21,000 Western Blot: 1:1000-1:2000Immunohistochemistry: 1:500-1:1500Optimal dilution determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:2mg
Host:goat
Source Antibody:rat
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate, 40% glycerol. Labeled with horseradish peroxidase (HRP).
Specificity:Recognizes rat IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, rat IgG and rat serum.