Immunoglobuoin G is a molecule of about 150kD composed of four peptide chains.
Each IgG contains two identical gamma heavy chains of about 51kD and two identical light chains of about 26kD, thus a tetrameric quaternary structure.
The two heavy chains are linked to each other and to a light chain each by disulfide bonds.
The resulting tetramer has two identical halves, which together form the Y-like shape.
Each end of the fork contains an identical antigen binding site.
The Fc regions of IgGs bear a highly conserved N-glycosylation site.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:20,000-1:500,000 has been assayed against 1ug of rat IgG in a standard capture ELISA using Streptavidin (HRP) and ABTS (A0030) as a substrate for 30 minutes at RT
Western Blot: 1:2000-1:10,000Immunohistochemistry: 1:1000-1:1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：1mg
Host：goat
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Labeled with Biotin.
Specificity：Recognizes rat IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-goat serum, rat IgG and rat serum.
