Applications:Suitable for Western Blot, ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000-1:4000ELISA: 1:3000-1:25,000Immunohistochemistry: 1:200-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
仕様
Size:1mg
Host:goat
Source Antibody:rabbit
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in 0.05M Tris-chloride, 0.15M sodium chloride, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.09% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (AP) (Calf Intestine MW:140kD).
Specificity:Recognizes rabbit IgG whole molecule. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-goat serum, rabbit IgG and rabbit serum.