Application:Suitable for Western Blot, ELISA, Immunohistochemistry and Immunomicroscopy.
Other applications not tested.
Recommended Dilution:ELISA:1:50,000-1:200,000
Western Blot: 1:5000-1:20,000
Immunohistochemistry: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:2mg
Host:goat
Source Antibody:rabbit
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography, followed by solid phase adsorption to remove any unwanted reactivities.
Form:Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 40% glycerol. Labeled with Biotin. Biotin/Protein Ratio: 10–20 BAC molecules per Goat IgG molecule.
Specificity:Recognizes rabbit IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-goat serum, rabbit IgG and rabbit serum.