Applications:Suitable for FLISA, Immunofluorescence and Western Blot.
Other applications have not been tested.
Recommended Dilutions:FLISA: 1:10,000-1:50,000Immunomicroscopy: 1:1000-1:5000Optimal dilution determined by the researcher.
Absorption Wavelength: 495nm
Emission Wavelength: 528nm
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
FITC conjugates are sensitive to light.
仕様
Size：1mg
Host：donkey
Source Antibody：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC). 3.0 moles FITC/mole IgG. Reconstitute in 1ml sterile ddH2O.
Specificity：Recognizes rabbit IgG, whole molecule. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-donkey serum, rabbit IgG and rabbit serum. Species Crossreactivity: Does not crossreact with bovine, chicken, goat, guinea pig, hamster, equine, human, mouse, rat and sheep serum proteins.
