Applications:Suitable for Immunoblot (Western or Dot blot), ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:5000Western Blot: 1:500-1:2500Immunohistochemistry: 1:200-1:1000Optimal dilution to be determined by the researcher.
Storage and Stability:Store at 4°C.
Do Not Freeze.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:1mg
Host:donkey
Source Antibody:rabbit
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.09% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (AP).
Specificity:Recognizes rabbit IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Donkey Serum, Rabbit IgG and Rabbit Serum.