Applications:Suitable for use in Western Blot, ELISA, Immunohistochemistry and Immunomicroscopy.
Other applications not tested.
Recommended Dilutions:ELISA: 1:20,000-1:100,000.1ug of Rabbit IgG was used in a standard capture ELISA using S7973-97A with ABTS as a substrate for 30 minutes at RT
Optimal dilutions to be determined by the researcher.
Biotin/Protein Ratio: 10–20 BAC molecules per Donkey IgG molecule
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：2mg
Host：donkey
Source Antibody：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with biotin.
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Donkey Serum, Rabbit IgG and Rabbit Serum.
