Applications:Suitable for use in FLISA and Immunofluorescence as well as other antibody based fluorescent assays.
Other applications have not been tested.
Recommended Dilutions:FLISA: 1:10,000-1:50,000Immunofluorescence: 1:1000-1:5000Optimal dilutions to be determined by the researcher.
Fluorochrome/Protein Ratio:4.1 moles FITC per mole of Sheep IgG
Absorption Wavelength: 495nm
Emission Wavelength: 528nm
Storage and Stability:Lyophilized powder is stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O prior to use.
To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：2mg
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC). Reconstitute with 2ml sterile ddH2O.
Specificity：Recognizes human IgG. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-sheep serum, human IgG and human serum
