Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, and Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
1ug of Human IgA using S7973-97A Streptavidin (HRP) and A0030 ABTS as a substrate for 30 minutes at RT.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Reconstitute to nominal volume by adding sterile 40-50% glycerol, ddH2O.
Aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, pH 7.4, 10mg/ml BSA (IgG and Protease free), 0.01% Thimerosal. Labeled with Biotin. Reconstitute with 1ml 40-50% glycerol.d dH2O.
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rabbit Serum, Human IgA and Human Serum. No reaction was observed against Human IgG or IgM. Specificity was confirmed at less than 1% against other human heavy or light chain isotypes by ELISA.
