Applications:Suitable for use in Immunofluorescence and FLISA.
Other applications have not been tested.
Recommended Dilution:IF Immunomicroscopy: 1:500-1:2500Optimal dilutions to be determined by the researcher.
Label:Fluorescein isothiocyanate (FITC)
Fluorochrome/Protein Ratio:2.6 moles FITC per mole of rabbit IgA
Absorption Wavelength:495nm
Emission Wavelength:528nm
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 6 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Prepared from monospecific antiserum by immunoaffinity chromatography using Human IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Form：FITC conjugated. Lyophilized in 0.02 M Potassium Phosphate, 0.15M sodium chloride, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Human IgA and Human Serum. No reaction was observed against Human IgG or Human IgM. Specificity was confirmed by ELISA at less than 1% crossreactivity against other Human heavy or light chain isotypes.
