Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Other applications not tested.
Recommended Dilution:ELISA: 1:40,000-1:190,000, using Peroxidase Conjugated Streptavidin, RT
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Form：Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin. Biotin/IgG ratio: 10-20 BAC molecules/Rabbit IgG molecule.
Specificity：Recognizes human IgM. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum, human IgM and human serum. No reaction was observed against human IgG or human IgA.
