Applications:Suitable for use in IFLISA, Western Blot and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Immunomicroscopy: 1:500-1:2500Optimal dilutions to be determined by the researcher.
Label:Fluorescein isothiocyanate (FITC)
Fluorochrome/Protein Ratio:4.9 moles FITC per mole of rabbit IgG
Absorption Wavelength:495nm
Emission Wavelength:528nm
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：500ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography using human IgM coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Form：Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with FITC.
Specificity：Recognizes human IgM, Fc. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-rabbit serum, human IgM and human serum. No reaction was observed against human IgG or human IgA.
