Fluorochrome/Protein Ratio: 2.5 moles FITC per mole of Rabbit IgG
Application(s): Suitable for use in FLISA and Immunofluorescence as well as other antibody based fluorescent assays.
Other applications have not been tested.
Recommended Dilution(s): Not specified.
Optimal titers for applications should be determined by the researcher.
Storage Conditions: Store vial at 4° C prior to restoration.
Restore with 1.0 ml of deionized water (or equivalent).
For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at-20° C or below.
Avoid cycles of freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4° C as an undiluted liquid.
Dilute only prior to immediate use.
Expiration date is one (1) year from date of restoration.
仕様
Size:1mg
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Form:FITC conjugated. Lyophilized in 0.02 M Potassium Phosphate, 0.15M sodium chloride, pH 7.2, 10 mg/ml Bovine Serum Albumin (BSA), IgG and Protease free, 0.01% sodium azide
Specificity:Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Human IgG, Human IgG F(ab’)2 and Human Serum. No reaction was observed against Human IgG F(c) or Mouse Serum Proteins.