Application(s): suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilution(s): This product has been assayed against 1ug of Human IgG in a standard capture ELISA.
A working dilution of 1:1,000 to 1:4,000 of the reconstitution concentration is suggested for this product.
Optimal titers for other applications should be determined by the researcher.
Storage Conditions: Store vial at 4° C prior to restoration.
Restore with 1.0 ml of deionized water (or equivalent).
For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at-20° C or below.
Avoid cycles of freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4° C as an undiluted liquid.
Dilute only prior to immediate use.
Expiration date is one (1) year from date of restoration.
仕様
Size：2mg
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Form：Lyophilized in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP).
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Human IgG and Human Serum. No reaction was observed against Mouse Serum Proteins.
