Applications:Suitable for use in FLISA, Fluorescent Western Blot, Immunofluorescence microscopy, Flow Cytometry and multiplex analysis.
Other applications have not been tested.
Recommended Dilution:FLISA: 1:10,000-1:50,000Immunofluorescence Microscopy: 1:1000-1:5000Flow Cytometry: 1:500-1:2500Optimal dilutions to be determined by the researcher.
Excitation: 495nm
Emission: 528nm
F/P Ratio: 3.6
Storage and Stability:Lyophilized powder is stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O prior to use.
To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：2mg
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (immunogloblin and protease free), 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC). Reconstitute with 1ml sterile ddH2O.
Specificity：Recognizes the heavy and light chains of human IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-rabbit serum, human IgG and human serum.No reaction is observed against mouse serum proteins.
