Applications:Suitable for use in ELISA, Western and Dot blot, Immunoperoxidase electron microscopy, Immunohistochemistry, as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency
Other applications have not been tested.
Recommended Dilutions:ELISA: 1:16,000-1:64,000.
Assayed against 1ug of human IgGin a standard capture ELISA using ABTS as a substrate for 30 minutes at RT.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：1mg
Host：goat
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Labeled with horseradish peroxidase (HRP). Reconstitute with 1ml 40-50% glycerol, ddH2O.
Specificity：Recognizes human IgG, Lambda. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. Crossreactivity: Other human heavy or light chain isotypes <1%.
