Biotin/Protein Ratio: 10–20 BAC molecules per Goat IgG molecule
Application(s): immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Recommended Dilution(s): This product has been assayed against 1ug of Rabbit IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin.
A working dilution of 1:12,000 to 1:50,000 of the reconstitution concentration is suggested for this product.
Optimal titers for other applications should be determined by the researcher.
Storage Conditions: Store vial at 4° C prior to restoration.
Restore with 1.0 ml of deionized water (or equivalent).
For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at-20° C or below.
Avoid cycles of freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4° C as an undiluted liquid.
Dilute only prior to immediate use.
Expiration date is one (1) year from date of restoration.
仕様
Size：2mg
Host：goat
Source Antibody：rabbit
Grade：Affinity Purified
Purity：Prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Form：Biotin conjugated. Lyophilized in 0.02 M Potassium Phosphate, 0.15M sodium chloride, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide
Specificity：Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Rabbit IgG, Rabbit IgG F(ab’)2 and Rabbit Serum. No reaction was observed against Rabbit IgG F(c) or Human Serum Proteins.
