Applications:Suitable for use in FLISA and Immunofluorescence as well as other antibody based fluorescent assays.
Other applications not tested.
Recommended Dilution:FLISA: 1:10,000- 1:50,000Immunofluorescence: 1:1000-1: 5,000Optimal dilutions to be determined by the researcher.
Label (FITC):Molecular Weight: 390DAbsorption Wavelength: 495nmEmission Wavelength: 528nmFluorochrome/Protein Ratio: 3.6 moles FITC per mole of IgG
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
FITC conjugates are sensitive to light.
仕様
Size:1mg
Host:goat
Source Antibody:rabbit
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Specificity:Recognizes rabbit IgG, F(ab')2. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-goat serum, rabbit IgG, rabbit IgG F(ab’)2 and rabbit serum. Does not crossreact with Rabbit IgG F(c) or human serum proteins.