Applications:Suitable for use in Western Blot, ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:4500Western Blot: 1:500-1:2500Immunohistochemistry: 1:200-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C before opening.
DO NOT FREEZE! Stable at 4°C as an undiluted liquid.
Dilute only prior to immediate use.
Stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
仕様
Size：1mg
Host：goat
Source Antibody：rabbit
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Form：Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.05% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (AP).
Specificity：Recognizes rabbit IgG. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine), anti-goat serum, rabbit IgG, rabbit IgG F(c) and rabbit serum. No reaction was observed against rabbit IgG F(ab’)2.
