Applications:Suitable for use in Immunoblot, ELISA, Immunohistochemistry and Immunomicroscopy.
Other applications have not been tested.
Recommended Dilution:ELISA: 1:10,000-1:40,000 30 min.
at RT.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at 4°C for short-term only.
Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:1mg
Host:goat
Source Antibody:rabbit
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Biotin. Reconstitute with 1ml sterile, distilled, deionized water or sterile 40-50% glycerol. Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule.
Specificity:Recognizes rabbit IgG. Assay by IEP resulted in a single precipitin arc against anti-biotin, anti-goat serum, rabbit IgG and rabbit serum. No reaction was observed against bovine, chicken, goat, guinea pig, equine, human, mouse, rat and sheep serum proteins.