Applications:Suitable for use in Dot Blot, Western Blot, ELISA, Immunoelectrophoresis and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500-1:3000ELISA: 1:2000-1:10,000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C before opening.
DO NOT FREEZE! Stable at 4°C as an undiluted liquid.
Dilute only pror to immediate use.
Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
AP conjugates are sensitive to light.
仕様
Size:1mg
Host:goat
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.09% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (Calf Intestine), MW: 140kD.
Specificity:Recognizes human IgG, IgA and IgM (all human immunoglobulin subclasses and chain combinations). Assay by IEP resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine) and anti-goat serum.